ANTIMICROBIAL RESISTANCE

The worldwide emergence of antibiotic resistance severely limits therapeutic options and is a significant global public health threat [1], [2].

Resistance to carbapenems in Enterobacterales can be caused by hyperproduction of AmpC β-lactamases or extended spectrum β-lactamases (ESBLs) combined with altered membrane permeability or by the production of carbapenemases (carbapenemase-producing Enterobacterales (CPE). Those enzymes are encoded mainly on plasmids and are highly transmissible.

The Ambler classification system categorizes β-lactamase enzymes into 4 Classes (A, B, C, D) based on their central catalytic domain and substrate preference [3].
Of these, classes A, B, and D include carbapenemases, whereas class C enzymes hydrolyze primarily cephalosporins. Enzymes in classes A, C, and D have serine in the active catalytic site, whereas class B enzymes are metallo-β-lactamases (MBLs) with zinc in the active site.

To contain the further dissemination of CPE, the rapid and accurate identification of carbapenemases is of paramount importance for both epidemiological and infection control purposes [4]. Rapid diagnostics [5] are one of the key points to tackle Antimicrobial Resistance. (Sir O’Neil, 2014, Antimicrobial Resistance: Tackling a Crisis for the Future Health and Wealth of Nations) [6].
NG Biotech’s objective is to propose a range of Lateral Flow Immunoassays (LFIA) which can compete with molecular techniques to transform the way we use antimicrobials in humans and animals: reducing unnecessary using, slowing AMR and so making existing drugs last longer.

NG Biotech provides RAPID diagnostic tests for the easy, fast, accurate detection of expressed Mechanisms of Resistance to Antibiotics (AMR) in (multi) drug-resistant bacteria (MDR)

Carba 5 Test

 

 

NG-Test® CARBA 5 is a multiplex lateral flow immunoassay for the rapid detection and differentiation of KPC, OXA-48, VIM, IMP and NDM in less than 15 minutes

 

 

NG-Test® Blood culture prep allows the Rapid and Direct detection of the major groups of carbapenemases from POSITIVE BLOOD CULTURE samples in CPE bacteria using the NG Rapid Test CARBA-5 .

 

 

NG-Test® CTX-M Multi is a multiplex lateral flow immunoassay for the rapid detection of the 5 most prevalent ESBL CTX-M Groups (1, 2, 8, 9 and 25) in less than 15 minutes

 

 

NG-Test® MCR-1 is a qualitative lateral flow immunoassay for the rapid detection of colistine resistance, in less than 15 minutes.

NG-Test® accurately identify the mechanism of resistance in less than 15’, which allows an earlier revision of the empirical regimen and a faster patient recovery.